cells with picogreen  (Yeasen Biotechnology)

Journal: STAR Protocols

Article Title: An optimized protocol to identify keratinocyte subpopulations in vitro by single-cell RNA sequencing analysis

doi: 10.1016/j.xpro.2022.101906

Figure Lengend Snippet: Pipetting map (A) Priming step. (B) Cell loading step. Figure is generated based on the Fluidigm’s protocol “Using C1 to Generate Single-Cell cDNA Libraries for mRNA Sequencing”. Protocol is available for download at https://www.fluidigm.com/support/instrument-support/c1-support .

Article Snippet: Single-Cell mRNA seq PicoGreen Template , Fluidigm , Cat# 100-6260.

Techniques: Generated, Sequencing

Journal: STAR Protocols

Article Title: An optimized protocol to identify keratinocyte subpopulations in vitro by single-cell RNA sequencing analysis

doi: 10.1016/j.xpro.2022.101906

Figure Lengend Snippet: Pipetting map for cell lysis, revere transcription, and PCR steps Figure is generated based on the Fluidigm’s protocol “Using C1 to Generate Single-Cell cDNA Libraries for mRNA Sequencing”.

Article Snippet: Single-Cell mRNA seq PicoGreen Template , Fluidigm , Cat# 100-6260.

Techniques: Lysis, Generated, Sequencing

Journal: STAR Protocols

Article Title: An optimized protocol to identify keratinocyte subpopulations in vitro by single-cell RNA sequencing analysis

doi: 10.1016/j.xpro.2022.101906

Figure Lengend Snippet: Output map Figure is generated based on the Fluidigm’s protocol “Using C1 to Generate Single-Cell cDNA Libraries for mRNA Sequencing”.

Article Snippet: Single-Cell mRNA seq PicoGreen Template , Fluidigm , Cat# 100-6260.

Techniques: Generated, Sequencing

Journal: STAR Protocols

Article Title: An optimized protocol to identify keratinocyte subpopulations in vitro by single-cell RNA sequencing analysis

doi: 10.1016/j.xpro.2022.101906

Figure Lengend Snippet: Output harvest map Figure is generated based on the Fluidigm’s protocol “Using C1 to Generate Single-Cell cDNA Libraries for mRNA Sequencing.”

Article Snippet: Single-Cell mRNA seq PicoGreen Template , Fluidigm , Cat# 100-6260.

Techniques: Generated, Sequencing

Journal: STAR Protocols

Article Title: An optimized protocol to identify keratinocyte subpopulations in vitro by single-cell RNA sequencing analysis

doi: 10.1016/j.xpro.2022.101906

Figure Lengend Snippet:

Article Snippet: Single-Cell mRNA seq PicoGreen Template , Fluidigm , Cat# 100-6260.

Techniques: Recombinant, DNA Library Preparation, Picogreen Assay, Software, Transferring, Cell Culture, Light Microscopy, Control, Fluorescence, Microscopy, Adhesive, Stripping Membranes

Journal: Gels

Article Title: Material Properties and Cell Compatibility of Photo-Crosslinked Sericin Urethane Methacryloyl Hydrogel

doi: 10.3390/gels8090543

Figure Lengend Snippet: DAPI (blue) and phalloidin (red) stained images of hMSCs seeded on TCP ( A ), SerAte-UM ( B ), and SerAte-M ( C ) hydrogels after three days of incubation in basal medium. Live (green) and dead (red) stained images of hMSCs seeded on TCP ( D ) or encapsulated in 15% wt SerAte-UM ( E ) and SerAte-M ( F ) hydrogels with 31% degree of modification after three days of incubation. The scale bar in ( A – F ) is 100 μm. Percent viability ( G ) and dsDNA content ( H ) of hMSCs seeded on TCP and encapsulated in 15 wt% SerAte-UM and SerAte-M hydrogels with 31% degree of modification as a function of incubation time. Error bars correspond to mean ±SD for n = 3.

Article Snippet: PicoGreen assay for quantification of dsDNA content of cells was purchased from Molecular Probes (ThermoFisher Scientific, Waltham, MA, USA).

Techniques: Staining, Incubation, Modification